Formation of collagen hydroxyproline in vitro

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Formation of collagen hydroxyproline in vitro.

Stetten & Schoenheimer (1943) fed [15N]proline to young rats and showed it to be a good source of the hydroxyproline of the carcass protein. Later, Stetten (1949) found that the efficiency of dietary [15N]hydroxyproline was less than one-twentieth of that of proline as a precursor of collagen hydroxyproline. These findings are of considerable interest since they are contrary to the general obse...

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Evolutionary Origins of C-Terminal (GPP)n 3-Hydroxyproline Formation in Vertebrate Tendon Collagen

Approximately half the proline residues in fibrillar collagen are hydroxylated. The predominant form is 4-hydroxyproline, which helps fold and stabilize the triple helix. A minor form, 3-hydroxyproline, still has no clear function. Using peptide mass spectrometry, we recently revealed several previously unknown molecular sites of 3-hydroxyproline in fibrillar collagen chains. In fibril-forming ...

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Objective- To determine the effect of collagen type I on hydroxyproline content in Achilles tendon in dogs. Design- Experimental in vivo study. Animals- A total of 12 adult dogs. Procedure- Under general anesthesia and aseptic condition the dorsal surface of right Achilles tendon was exposed and after complete separation of  connective tissue, it was splitted (striking 10 times) in full thic...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1959

ISSN: 0306-3283

DOI: 10.1042/bj0710055